The Lysogenic Cycle: Viral Dormancy and Integration
Bacteriophage lambda can switch between two distinct life cycles: the lytic cycle and the lysogenic cycle.
The lysogenic cycle
Step-By-Step Breakdown Of The Lysogenic Cycle
Step 1: Attachment
Bacteriophage λ attaches to E. coli using tail proteins that bind specifically to maltoporin, a protein in the bacterial outer membrane.
Step 2: DNA Entry (Penetration)
The phage contracts its tail sheath like a syringe, injecting viral DNA into the bacterial cytoplasm.
The viral DNA enters as a linear molecule with sticky ends, which rapidly circularizes inside the host.
Only the DNA enters, the capsid remains outside.
Step 3: Integration into Host Genome
The enzyme integrase (encoded by the phage) inserts viral DNA into a specific site in the bacterial chromosome.
Once integrated, the viral DNA is called a prophage.
Step 4: Host Cell Division (Prophage Replication)
The bacterial cell continues normal growth and reproduction.
The prophage DNA is replicated passively alongside host DNA.
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Question 1
Recap question
In a population of E. coli carrying a lambda prophage under non-stress conditions, what mechanism ensures each daughter cell inherits the viral genome?
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What are the two life cycles of bacteriophage lambda?
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Note
Introduction to Bacteriophage Lambda
Bacteriophage Lambda is a virus that infects Escherichia coli bacteria.
It can switch between two life cycles: the lytic cycle and the lysogenic cycle.
This phage is a key model for studying virus-host interactions and gene regulation.
DefinitionBacteriophage LambdaA virus that infects bacteria, specifically E. coli, and can alternate between lytic and lysogenic life cycles.
AnalogyThink of bacteriophage lambda as a spy that can either launch an attack (lytic cycle) or go undercover (lysogenic cycle) within its host.
NoteBacteriophage lambda is a temperate phage, meaning it can choose between lytic and lysogenic cycles based on environmental conditions.